On February 9th, over 55 in-person and 60 online attendees joined a collaborative symposium to explore the impact of AI in research and practice on AMR. The event, co-organized by Imperial College London’s Institute of Infection, the AI Network, the Centre of Antimicrobial Optimisation, and the NIHR Health Protection Research Unit for Healthcare-Associated Infections and Antimicrobial Resistance, showcased the breadth of research being undertaken across Imperial and brought researchers together from different disciplines. In sessions focused on different parts of the translational pipeline, examples were presented of how AI models are being used for AMR prediction and clinical decision support. Future opportunities and barriers were also explored.